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KMID : 0903519920350060507
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1992 Volume.35 No. 6 p.507 ~ p.512
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Effects of benzyladenine on the cell wall regeneration of soybean(Glycine max) protoplasts
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Abstract
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A β-1,3-glucanase of soybean (Glycine max) was isolated, and the effects of benzyladenine(BA) on celluar levels of the enzyme content and activity were studied. The effects of BA on callow content in cell wall and wall regeneration of protoplasts were also studied to show promoting effect of cytokinin in cell wall regeneration and to elucidate action mode of cytokinin. The polypeptide of 21 kD was identified as β-1,3-glucanase, and the cellular content and activity of this polypeptide were decreased by BA treatment. The callose content in cell wall of callus and the wall regeneration of protoplasts were increased by BA treatment. These results indicate that cytokinin promotes cell wall regeneration by inhibition of callose degradation via decreasing β-1,3-glucanase level in cell.
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